![]() ![]() The number of CTCs increased with the progression of NCCN TNM stages and showed statistically significant difference between stage I and later stages. CTCs are enumerated from blood samples of patients suffering lung carcinoma. We demonstrate that the WBC capture efficiency is as high as 97.0%, and the recovery rate of cancer cells reaches up to 97.5%. Through the specific biotin-streptavidin interaction, the device is able to sort out biotinylated anti-CD45 antibody-labeled white blood cells (WBCs) and enrich A549 human cancer cells from the blood or CTCs from patient suffering non-small cell lung cancer. In this study, a negative sorting method has been developed for isolation of CTCs through a microfluidic platform integrated with streptavidin-functionalized electrospun polylactic-co-glycolic acid nanofibers. ![]() However, isolated CTCs, especially those captured by positive sorting methods, are difficult to culture in subsequent assays because the cells have to be labeled or attached to a substrate for separation. Detection of circulating tumor cells (CTCs) plays an important role in early diagnosis of cancer and personalized therapy.
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